The article presents a unique copy of the 1601 edition of Hierosolymitana peregrinatio (‘The Journey to Jerusalem’) by Nicholas Christopher Radziwiłł the Orphan currently preserved at the library of the Lithuanian Institute of History in Vilnius. Specific corrections left in margine prove the proofreading nature of this copy. It is asserted that the second Latin edition of the ‘Journey’ printed in Antwerp by the Plantin printing house in 1614 was prepared and published on the basis of the aforementioned copy. Unpublished letters stored in the archival collections of the Plantin-Moretus Museum reveal a completely unknown history of the printing process of the second Latin edition of the ‘Journey’. All these letters are published in the appendix to the article in their original language (Latin) with comments and translation into Lithuanian.