The article presents scientific, pedagogical and collecting activities of priest Stanisław Boniface Jundziłł (1761–1847) – botanist, professor of natural history at the University of Vilnius in the end of 18th-begining of 19th century. Foreign nature studies in Vienna under the supervision of professor Joseph Franz von Jacquin, which he held in the years 1792–1797, were an important period in the scientific development of Jundziłł. During this period, he gained not only theoretical knowledge, but also, among others, he carried out numerous herborizations, traveled and visited collections, gardens and mines. At that time, Vienna was one of the most important scientific centers in Europe dealing with natural sciences – botany, zoology and mineralogy. After returning to Vilnius, Jundziłł started teaching, and in 1799 he was appointed the main guardian of the university collections: the botanical garden and the natural history cabinet. Thanks to the Vilnius botanist, these collections have become educational and research spaces at a high European level.